Hitachi U 2000 Owners Manual

							Hitachi Double Beam 
Spectrophotometer 
						

							1
Model 124 
Model U-2000
Spectrophotometer simpler to use and 
higher in dependability thanks to mounting
of a large-size color LCD.  
High resolution satisfying European Pharmacopoeia (spectral bandpass: 1.5nm).  
Trace-amount measurement in biotechnological field, etc. can be carried out using optional 50, 25
and 5mL micro-volume cells. 
■An abundance of optional accessories for various applications have been lined up.  
■Function for automatic data storage in a USB memory is incorporated.  
■Validation function and self-diagnostic function are standard with this instrument.   
■The UV Solutions control program available at option enables the user to control 
operations from a personal computer.   
(The Model U-2910 is a special
model for PC control.) 
*:  Available at option 
Model 100-50
Model U-2001Model U-2800 Model 150-20 
Spectral bandpass 1.5nm satisfying European Pharmacopoeia 
European Pharmacopoeia requires a ratio of 1.5 or larg-
er when measuring 0.02% (V/V) solutions of toluene in
hexane.  In the spectrum at right, a satisfactory value of
1.6 can be confirmed.  
(Sample: 0.02% solution of toluene in n-hexane) 
2
Stable optics due to double beam  
Incorporation of stigmatic concave diffraction grating
The optics of this instrument adopts the Seya-Namioka monochromator widespread as a representative concave
diffraction grating monochromator.  
Because a concave diffraction grating has both beam condensing and dispersing functions, an optical system can
be configured with fewer mirrors.  In a spectrophotometer, use of fewer mirrors signifies a shorter optical path,
thus giving rise to an aberration-free bright optics.  
For elimination of the aberrations which were essentially unavoidable in the past, a stigmatic concave diffraction
grating has been developed by applying Hitachi’s original technology.  As a result, a higher resolution has been
realized. 
Detector 1  Mirror D
2 lamp
WI lamp Concave diffraction
grating
Entrance 
slit
Exit slit 
Filter
Mirror
MirrorHalf
mirror
Reference 
side
Sample 
side
MonochromatorSample 
compartmentLens
LensDetector 2
In double beam design, the energy of the light source is divided into two with
a half mirror so that one passes through the reference side, and the other
through the sample side, which is unavailable with the single beam design.  
Since the reference-side energy is also incident on a detector, photometry is
carried out on the basis of this signal.  Therefore, an energy change in the
light source can be compensated to ensure stable measurement for a long
time. 
A higher resolution has been achieved
by eliminating coma from the Seya-
Namioka monochromator which is the
most popular concave diffraction grat-
ing monochromator.  Its grooving is
supported by the only ruling engine in
Japan.  The diffraction gratings of
Model U-2900/2910 have also been
made with this machine.  
						

							3
Mounting of 26.4cm color LCD, the largest 
in this product class 
Due to a 640 ×480 color LCD with backlight, working curves, spectra and other minute displays are easier to view, and charac-
ters are bigger than before, which ensures a high visibility.  A dedicated keyboard (for U-2900 alone) is quite helpful for baseline
measurement and other operations. 
*  The size in the photo is 1/2 of the actual LCD size. 
*  The photo size is 1/2 of the actual keyboard size. 
4
Introduction of Measurement Modes 
Photometry
The concentration of an unknown sample can be deter-
mined 
in comparison with the known concentration of a standard
sample. 
The linear/quadratic regression curve or polygonal line
approximation curve is drawn by measuring plural standard
samples (max. 20 samples) to calculate the concentration. 
In addition, judgment of the upper and lower concentration
limits can be set and quantitative analysis can also be car-
ried out via factor input. 
Wavelength scan
A spectrum resulting from scanning over a desired wave-
length range within 190 to 1,100nm can be displayed. 
Because each substance has a unique spectrum, its char-
acteristics can be examined. 
After measurement, peak search, smoothing and other
data processing can be effected.  And, a repeat scan capa-
bility helps the user trace a chemical reaction process.
Baseline correction is also available. 
Time scan
A spectrum can be drawn by following a change of photo-
metric value at a single wavelength with the lapse of time.
This function is used for analysis of enzymatic reactions.
Enzyme activity is measured according to a change of
absorbance within the set time period.  Peak detection,
smoothing, kinetic assay and other data processing can be
performed. 
Multiple-wavelength measurement
Purity of a nucleic acid can be calculated using a ratio of
absorbance values at 2 wavelengths (A260/A280). 
Furthermore, measurement can be performed while auto-
matically shifting measuring wavelength to a maximum of 6
wavelengths.  This is convenient when only measured data
at each wavelength is desired.  
						

							6
Easy to Save Measured Data.  
Measured data can be saved in a commercially available USB flash memory*. 
The data is savable in the text file format. 
A commercial spreadsheet program can processing the measured data. 
* Not all of the commercial USB flash memory products are usable.  Contact us separately. 
Data saving with USB flash memory
Data can be transferred easily to PC by means of a USB flash memory. 
Data loading
Because the measured data is saved in the text file format,
it is sent directly to a commercial spreadsheet program. 
This function can be utilized for detailed data analysis,
report generation, etc.
Printout of data from 
spectrophotometer
The data measured with the spectrophotometer including
spectra and working curve graph can be printed with a
small-sized thermal printer (DPU) separately available.
* Contact us separately.
5
Highly dependable system incorporating auto-
matic calibration and self-diagnostic function. 
A highly dependable system was designed by incorporating lots of functions for memory
check, wavelength drive check, lamp ignition check, automatic wavelength calibration, lamp
ignition time display, etc. 
(This function is standard with the Model U-2900 main unit.  The Model U-2910 provides the equivalent functions via PC control.) 
Validation function
This is a function for checking the performance of Model
U-2900/2910 main unit.
The following items can be checked. 
●Wavelength accuracy ●Baseline flatness 
●Wavelength setting repeatability ●Baseline stability 
●Bandpass ●Noise level 
Example printout 1 of validation function
with dot impact printer (for 1 check item) 
Example printout 2 of validation function with
dot impact printer (covering all check items) 
Automatic calibration and 
self-diagnostic function
Upon initialization, memory check, wavelength drive mech-
anism check, lamp ignition check and 656.1nm check are
automatically diagnosed and adjusted. 
Also, the automatic wavelength calibration function is
incorporated for automatic calibration by using the bright
line of D
2lamp as a reference wavelength. 
Besides, each cumulative ignition time of the WI and D
2
lamps can be displayed.  This function is useful for instru-
ment management.  
						

							8
Instrument application further expandable
by UV Solutions program. 
Option package program (P/N 2J1-0311)
Capable of performing color calculation and color difference calculation.
Nucleic acid measurement program (P/N 2J1-0316)
Usable for checking extraction and refinement of nucleic acids such as DNA/RNA essential for genetic research.  Data (photo-
metric values at 230, 260 and 280nm), and calculation results (260/280, nucleic acid concentration, protein concentration,
molar concentration) can be displayed collectively on the screen. 
U-2910 is a special model for PC control. 
GLP/GMP program (P/N 2J1-0317)
The burden of optical instrument performance check can be alleviated. 
Item-dependent measurement mode and automatic measurement mode (when no sample is necessary) have also been pre-
pared.  In addition, a judgment tolerance range is settable so as to meet each analytical purpose. 
Report generator program (P/N 2J1-0312)
Developed to enable freely customizing the report output format of measurement results.  Each character size and position of
report items and comment are settable and besides, mathematical calculations conventionally dependent on manual method
can be executed by spreadsheet program functions. 
UV Navigation program (P/N 2J1-0313)
Provides support for automation of measurement, data processing and printout.
Measuring procedure can be readily programmed using a graphical editor for imple-
mentation of spectrophotometer control, measurement result indication and judg-
ment, etc.  Because analytical procedure can be automated, it is possible to save
labor and form working patterns.  This is effective for routine work such as quality
control. 
7
Personal computer-based instrument 
control and data processing.
The Model U-2900 becomes controllable from PC when the
UV Solutions program (P/N 2J1-0310) is installed.   Not only
the U-2900 functions such as photometry and wavelength
scan, but also data pasting to another application program,
data transfer, report generation, etc. will be supported. 
(The Model U-2910 is a special instrument for PC control. 
A PC compatible with Windows
®XP needs to be prepared separately.) 
Simple to use
Buttons are laid out so as to follow the operating proce-
dure.  Hence, operations can be performed smoothly.  
A rich variety of data processing functions
Spectrum can be expanded, contracted, smoothed, differ-
entiated and integrated, and fundamental arithmetic calcu-
lations are applicable between spectra. 
Powerful support for report generation
Measured data can be sent to Microsoft®Excel.  One click
of the Report button suffices for data transmission. A spectrum can be pasted to another application program. 
Print preview
Before printing, the contents can be checked by the Print
Preview command. 
Measured data text convertible
Besides conversion into
the ASCII text or J-
CAMP format, spectra
can be saved in Metafile
format. 
Windows®XP and Microsoft®Excel are trademarks of Microsoft Corporation, U.S.A. 
Microsoft®and Windows®are registered trademarks of U.S. Microsoft Corporation in USA
and other countries.  
						

							10
Micro cell holder  
(P/N 122-0060)
Suitable for measurement of trace samples in
medical and biochemical fields. 
Specifications (cell required separately) 
Wavelength range 220 to 950nm 
Cell mounting
/demounting repeatabilityWithin ±0.3%T
Baseline flatnessWithin ±0.005Abs 
(50mL micro-volume cell used) 
The following cells are usable for the above micro
cell holder (P/N 122-0060). 
Tandem cell holder 
(P/N 210-2115)
Up to three 10mm cells can be mounted on each
of the sample and reference beam sides.  Sample
temperature can be maintained at a constant level
by circulating constant-temperature water through
the cell holder section. 
Specifications (circulating thermostatic chamber
and cell not included in this product)
Control temperature range 15 to 40°C 
Temperature stability ±0.3°C 
Five 10mm rectangular cells can be mounted on
the sample beam side and a micro cell mask (P/N
200-1537) can be inserted in each cell holder.
(Cell and micro cell mask are not included in this
product.)  It is recommended to prepare a set of
five cells. 
Recommended cell sets  
124-0352 10mm quartz cell set (5 cells in set) 
124-0378 10mm glass cell set (5 cells in set) 
Four rectangular long path absorption cells can be
mounted on the sample beam side and they can be
changed over externally. 
Specification  
Cell length 100mm, 50mm to 10mm cells applicable 
Film holder 
(P/N 210-2112)
Designed for measurement of film-like samples. 
Specifications  
Film frame Width 25mm, height 30 to 50mm 
Beam aperture Width 10mm ×height 20mm 
Polarizer holder  
(P/N 210-2130)
Used for linearly polarizing the sample beam to
check its polarization characteristic and for meas-
uring optical rotary power with a sample placed
between the polarizer and analyzer. 
Specifications  
Wavelength range 400 to 750nm Sample thickness 0.5 to 5mm 
Sample size Min. 12 ×25mm/max. 55 ×100mm 
Mask for Micro cell
To be inserted into a standard rectangular cell
holder for measurement of a trace sample. 
Optical path lengths measured at 21 points on cell
using a three-dimensional measuring instrument
are indicated down to the fourth decimal place
(mm). 
Communication cable  
(P/N 121-1521)
A computer and the spectrophotometer main unit
need to be connected with this cable for computer
control of measurement using the UV Solutions
program. 
Specifications  
200-1537 Mask for Micro cell (1.5mm) 
200-1538 Mask for Micro cell (1.2mm)
Cell
124-0357 Micro quartz cell, 10mm 
200-0551 Black quartz micro cell, 10mm
Rectangular long path cell holder 
(P/N 210-2107)
Specifications
Cell capacity 8µL Optical path length 8mm (quartz flow cell used) 
Baseline flatness±0.001Abs (200 to 350nm) 
±0.002Abs (190 to 850nm) 
Part No. Part name CapacityOptical path length 130-0622 50 micro cell 50µL 10mm130-0623 25 micro cell 25µL 5mm
130-0621 5 micro cell 5µL 0.5mm
Conditions of applicable rectangular cell are
given below.
Optical path length 10, 20, 30, 40, 50, 100mm 
Outer width 12.75nm
4-Position rectangular 
long path cell holder    
(P/N 150-0940)
Used for transmittance/absorbance measurement
of such a solid sheet sample as glass filter. 
Glass filter holder     
(P/N 210-2109)
Specifications  
Sample thickness 0.5 to 5mm 
Sample size 12 ×25mm to 55 ×100mm 
Used for measurement with a cylindrical cell. 
Cylindrical long path cell holder
(P/N 210-2108)
5-Position turretcell holder
(P/N 210-2110)
Examined 10mm 
rectangular quartz cell
(P/N 210-1462)
9
Diverse optional accessories selectable for specific
applications such as multiple samples and trace sample. 
Auto sipper   
(P/N 2J1-0100)
This computer-controlled sample sipper is provid-
ed with a sample recovery function and other ver-
satile functions.  In combination with an autosam-
pler, this unit will further advance automation and
labor saving in the preparatory stage.  This sipper
cannot control temperature. 
Specifications 
Minimum sample volume 0.6mL Carryover 1% or less Cell capacity About 50µL Optical path length 10mm 
Reference beam side 10mm rectangular cell mountable 
Water circulated cell holder    
(P/N 210-2111)
Water from a thermostatic chamber is circulated
through this cell holder to maintain a sample cell at
a constant temperature. 
Specifications(circulating thermostatic chamber and cell not included in this product)
Operating temperature rangeFrom normal temperature to 40°C 
Temperature stability Within ±0.3°C 
Micro flow cell unit 
(P/N 210-2113)
Suitable for continuous measurement of a trace
sample. 
Specifications 
Difference in capacity due to cell changeoverWithin ±0.5% (at 100%T) Applicable cell10mm cell (not included in this product) Settable temperature 20 to 40°C 
Designed to have the same structure as the above
auto sipper.  Because the flow cell section can be
maintained at a constant temperature, exact con-
trol is ensured. 
Specifications(without temperature control on reference side) 
Minimum sample volume 0.6mL Carryover 1% or less Cell capacity About 50µL Optical path length 10mm Settable temperature 20 to 40°C 
Reference beam side 10mm rectangular cell mountable 
Specifications
Cell capacity 70µL Optical path length 10mm (quartz flow cell used) Withstand pressure Max. 0.1MPa 
Connecting tubeTeflon tube. Outer diameter 2mm, 
inner diameter 1mm 
Flow cell unit  
(P/N 210-2173)
The inside of this cell is structured to minimize
stagnation of liquid and adhesion of air bubbles. 
Specifications
Cell capacity 600µL Optical path length 5mm (quartz flow cell used) Withstand pressure Max. 0.1MPa 
Connecting tube Teflon tube.  Outer diameter 4mm, 
inner diameter 3mm 
Reference beam side5mm rectangular cell (standard accessory) 
LC flow cell unit   
(P/N 210-2131)
Exclusively used for a liquid chromatograph.  In
wavelength scan, the baseline is always kept flat. 
Electronic thermostatted
cell holder 
(P/N 131-0306/0307)
Standard-equipped with a magnet stirrer to main-
tain a constant sample temperature in each cell.
Temperature can be indicated down to a minimum
0.01°C scale.  Because of an electronic thermostat-
ted type using forced air cooling, this cell holder is
capable of quick heating and cooling.  A thermo-
static chamber is unnecessary.  
(Temperature control: S only) 
Specifications
Control temperature range10° to 60°C (settable in steps of 0.01°C) (normal temperature 25°C)Temperature setting accuracy Within ±2°C*(difference between set temperature and sample temperature) Temperature stability Within ±0.5°C*
* Room temperature:  25°C, sample:  distilled water 
In the measurement of protein or nucleic acid
fusion, a sample temperature can be changed con-
tinuously to determine variation in absorbance.
Being of an electronic thermostatted type, this cell
holder is capable of quick heating and cooling.
Sample temperature can be increased and
decreased isothermally.  In addition, the set tem-
perature can be maintained evenly inside a cell,
because a stirrer is provided. 
(Temperature control: S and R) 
Specifications
Control temperature range0° to 100°C (settable in steps of 0.1°C)(normal temperature 25°C) Temperature setting accuracyWithin ±2°C* (difference between set temperature and sample temperature)Temperature stability Within ±0.5°C* 
Equipped with isothermal temperature regulation function 
* Room temperature:  25°C, sample:  distilled water, circulatory
water temperature: 22°C, set temperature: 10 to 60°C  
A circulating thermostatic chamber needs to be prepared separately.
AS-1010 Auto sampler
(P/N 2J1-0121/0122)
In combination with the auto sipper or in flow
injection analysis, this unit is used for multiple-
sample measurement.  A suction needle can be
moved in three directions X, Y and Z. 
Six 10mm cells can be mounted on the sample
beam side, and they can be changed over automat-
ically at certain intervals.
Specifications(sample tube not included)
Outer diameter 15mm, height 105mm 
Sample tube size (option required) 
Outer diameter 12 mm, height 105 mm 
6-cell positioner with
temperature control
(P/N 2J1-0103/0104)
Auto sipper with 
temperature  control 
(P/N 2J1-0101)
Electronic thermostatted
cell holder 
(Thermostatted water bath is need-
ed separately)(P/N 131-0301/0302) 
						

							NOTICE: For proper operation, follow the instruction manual when using the instrument.
Specifications in this catalog are subject to change with/or without notice, as Hitachi High-Technologies Corporation continues to develop the latest 
technologies and products for our customers.
Tokyo, Japan
http://www.hitachi-hitec.com/science/
24-14 Nishi-Shimbashi 1-chome, Minato-ku, Tokyo, 105-8717,  Japan
Tel: +81-3-3504-7211   Fax: +81-3-3504-7302
Printed in Japan (H) HTB-E036P2007.2