Hitachi F 2500 Manual

2 - 8 
(l) EM Slit  
Select a slit width for the emission side.    Unit:    nm   
 
F-4500 F-2500  
1.0 2.5  
2.5 5.0 
5.0 10.0 
10.0 20.0 
20.0  
 
 
(m) PMT Voltage  
A function for controlling the voltage of the 
photomultiplier detector (photomultiplier tube).     
Select one of the following values.    Changing from 
400 V to 700 V increases the data value about two 
digits and changing from 700 V to 950 V increases it 
about 1 digit.     
  950 V (only with F-4500)   
 700 V  
 400 V 
 
(n)...

2 - 9 
(o) Corrected spectra  
A function for determining the spectrum inherent to a 
sample by correcting the photometer wavelength 
characteristic using the saved instrument parameters, 
following measurement with the instrument parameters 
for photometer control.    If there are no instrument 
parameters for the wavelength range to be measured, 
only the wavelength range that allows correction is 
scanned.    And if there is no wavelength area 
measurable, an error will occur.     
For using this...

2 - 10 
(3) Monitor  
 
When selecting the Monitor tab, the window in Fig. 2-5 
appears.   
 
 
 
Fig. 2-5  Monitor Tab 
 
 
Assign the following parameters.   
 
(a)  Y-Axis Max (maximum)   
Input a maximum value for Y-axis on the monitor 
window.  
 
(b)  Y-Axis Min (minimum)   
Input a minimum value for Y-axis on the monitor 
window.  
 
(c)  Open data processing window after data acquisition   
Select whether or not to conduct data processing after 
sample measurement.    When selected (check mark is...

2 - 11 
NOTE:  When the remaining memory capacity of PC 
in operation is very small (system resource 
is less than 20%), the data processing 
window will not open even if this item is 
turned ON.    Either close the presently 
opened data processing window or restart 
the FL Solutions program.     
 
(d)  Print report after data acquisition   
Select whether or not to print a report after sample 
measurement.    When selected (check mark is 
applied), printing will be made automatically after...

2 - 12 
(a)  CAT (Computing for Averaging Transient)   
Used for obtaining an average spectrum in repeat 
measurement.    This is especially effective for 
measuring samples with which the emission intensity 
varies with time.   
 
(b) Processing choices  
A list of data processing (Savitsky-Golay, Mean 
smooth, Median smooth, Derivative) is shown.     
Select a data processing item, and click the rightward-
pointing arrow key between the Processing choices 
and Processing steps display fields.    Then,...

2 - 13 
(5) Report  
 
Select the Report tab, and the window in Fig. 2-7 will 
appear.  
 
 
 
Fig. 2-7  Report Tab 
 
 
This window is used to select an output method and items 
of measurement result.     
 
(a) Output  
1) Print Report  
A report will be printed.    For printing method, 
refer to 2.6.   
2)  Use Microsoft® Excel   
Data will be transferred to Microsoft® Excel.     
For transfer method, refer to 5.13.   
3)  Use print generator sheet 
Select this method for using the report generator...

2 - 14 
(c)  Printable (transferable) items   
1) Include date  
2) Include method  
3) Include graph  
4)  Include data listing   
 Constant  
Data will be printed at fixed intervals between 2 
wavelength points.    Input Data interval, Start 
wavelength and End wavelength.     
 Select data 
Data at the specified wavelength will be printed.   
Up to 12 wavelengths can be specified.   
5)  Include peak table 
Select output items in the peak table.   
  Peak WL/Peak data   
  Start WL/End WL   
...

2 - 15 
2.3  Defining Your Samples  
 
When “Use Sample Table” has been set for the Analysis Method, 
the samples must be defined before measurement.   
 
(1)  Open the monitor window and click the          button.   
 
(2)  When the Sample Table window in Fig. 2-8 appears, fill in 
the blank section.    For instance, if there are five samples, 
click the Insert button five times.   
 
 
 
Fig. 2-8    Sample Table Window 
 
 
(3)  Input the Sample, Comments and File Name for each 
sample.    The sample...

2 - 16 
(4)  When input is finished, check the entries and then click the 
OK button.   
If a sample table is not used, then click the          button.   
The window in Fig. 2-9 will appear.    Make entries for 
Sample and Comment.    For automatic saving, put a check 
mark in the “Auto File” box and specify a file name for 
saving.  
 
 
 
Fig. 2-9 
 
2.3  Defining Your Samples  

2 - 17 
2.4  Prescan  
 
Select Pre-scan command from the Spectrophotometer menu or 
click the     (pre-scan) button.  
This function executes high-speed scan under the set measuring 
conditions, detects the peak wavelength and moves the system 
to that wavelength.    Also, the upper limit photometric value is 
automatically set so that the peak-wavelength value becomes 
approximately 70% of that upper limit value.     
Scan ranges are as follows.   
 
Excitation spectra  :  Start wavelength to emission...