Hitachi F 2500 Manual

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(f) Delay  
After pressing the Measure button, measurement is 
started following the delay time set here.    It is used for 
temperature stabilization, etc.    In repeat measurement, 
it is the time until the start of the first measurement.   
The delay time is invalid for the second and 
subsequent measurements.   
Input range:    0 to 9999 s   
 
(g) EX Slit  
Select a slit width for the excitation side.    Unit:    nm   
 
F-4500 F-2500 
1.0 2.5  
2.5 5.0 
5.0 10.0 
10.0 20.0 
20.0  
 
 
(h) EM Slit  
Select a slit width for the emission side.    Unit:    nm   
 
F-4500 F-2500 
1.0 2.5  
2.5 5.0 
5.0 10.0 
10.0 20.0 
20.0  
 
 
(i) PMT Voltage  
A function for controlling the voltage of the 
photomultiplier detector (photomultiplier tube).     
Select one of the following values.    Changing from 
400 V to 700 V increases the data value about two 
digits and changing from 700 V to 950 V increases it 
about 1 digit.     
•  950 V (only with F-4500)   
• 700 V  
• 400 V 
 
3.2  
						

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(j) Response  
Select a response time from the following.   
 
F-4500 F-2500 
0.04 0.04 
0.01 0.08 
0.05 0.4 
0.1 2 
0.5 8 
2.0 Auto 
8.0  
Auto  
 
 
By selecting Auto, a response time is set automatically 
according to scan speed.    Select Auto usually.   
 
(k)  Shutter control (only with F-4500)   
The shutter can be automatically closed in other than 
measurement for minimizing sample deterioration due 
to the energy of excitation beam and opened when 
measurement starts.    When you put a check mark at 
the head, the shutter will close and open at start of 
measurement.    The shutter will close again when 
measuring wavelength begins returning to the start 
wavelength after measurement in the time scan mode.     
 
(l) Replicates  
Set the number of repeat measurements.    Input range 
is 1 to 99.     
 
(m) Cycle time 
Set a repetition interval.    Input range is 0.0 to   
180.0 min.   
 
3.2    Creating an Analysis Method  
						

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(3) Monitor  
 
When selecting the Monitor tab, the window in Fig. 3-5 
appears.   
 
 
 
Fig. 3-5  Monitor Tab 
 
 
Assign the following parameters.   
 
(a)  Y-Axis Max (maximum)   
Input a maximum value for Y-axis on the monitor 
window.  
 
(b)  Y-Axis Min (minimum)   
Input a minimum value for Y-axis on the monitor 
window.  
 
(c)  X-Axis Max (maximum)   
Input a maximum value for X-axis on the monitor 
window.  
 
(d)  X-Axis Min (minimum)   
Input a minimum value for X-axis on the monitor 
window.  
 
NOTE:  X-Axis Max and Min correspond to a display 
interval on the monitor window.   
For example, when Max and Min are 60 and 
30, display on the window is made within 30 
to 60 s, 60 to 90 s, 90 to 120 s and so on.       
3.2  
						

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(f)  Open data processing window after data acquisition   
Select whether or not to conduct data processing after 
sample measurement.    When selected (check mark is 
applied), data is automatically saved after 
measurement.  
 
NOTE:  When the remaining memory capacity of PC 
in operation is very small (system resource is 
less than 20%), the data processing window 
will not open even if this item is turned ON.   
Either close the presently opened data 
processing window or restart the FL 
Solutions program.   
 
(g)  Print report after data acquisition   
Select whether or not to print a report after sample 
measurement.    When selected (check mark is 
applied), printing will be made automatically after 
measurement.    The items set under the Report tab 
will be printed.     
 
(h) Overlay  
Spectra can be overlaid in the monitor window.     
This setting cannot be selected for repeat 
measurement.   
 
(4) Processing  
 
On selection of the Processing tab, the window in Fig. 3-6 
will be displayed.   
 
 
 
Fig. 3-6  Processing Tab 
3.2    Creating an Analysis Method  
						

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(a) Processing choices  
A list of data processing (Savitsky-Golay smooth, 
Mean smooth, Median smooth, Derivative) is shown.     
Select a data processing item, and click the rightward-
pointing arrow key between the Processing choices 
and Processing steps display fields.    Then, the 
selected method appears in the Processing steps field.   
 
(b) Processing steps  
The processing sequence is displayed.    To delete a 
processing method, first select the method, and then 
click the leftward-pointing arrow key between the 
Processing choices and Processing steps display 
fields.    Then, the selected method disappears from 
the Processing steps field.   
Also, in case you want to carry out any smoothing in 
Processing steps, double-click the relevant item and 
the smoothing parameters (order, data points, etc.) can 
be changed.   
 
(c) Peak Finding  
Set threshold and sensitivity values for peak detection.   
 
(d) Kinetics  
Input a start time, end time and K-factor for kinetics 
(rate calculation).   
 
3.2  
						

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(5) Report  
 
Select the Report tab, and the window in Fig. 3-7 will 
appear.  
 
 
 
Fig. 3-7  Report Tab 
 
 
This window is used to select an output method and items 
of measurement result.     
 
(a) Output  
1) Print Report  
A report will be printed.    For printing method, 
refer to 3.6.   
2)  Use Microsoft® Excel   
Data will be transferred to Microsoft® Excel.     
For transfer method, refer to 6.13.   
3)  Use print generator sheet 
Select this method for using the report generator 
(option).    For usage, refer to the instruction 
manual of the report generator.   
 
(b) Print format  
Selectable when Report is chosen for Print Method.   
Select a printing orientation.    Either Portrait or 
Landscape orientation is selectable.    When the 
Landscape orientation is selected, date and method 
can be printed.   
 
3.2    Creating an Analysis Method  
						

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(c)  Printable (transferable) items   
1) Include data 
The date and time of analysis can be printed.   
Printing time will always be printed.       
2) Include method  
3) Include graph  
4)  Include data listing   
• Constant  
Data will be printed at fixed intervals between 2 
wavelength points.    Input Data interval, Start 
wavelength and End wavelength.     
• Select data  
Data at the specified wavelength will be printed.   
Up to 12 wavelengths can be specified.   
5)  Include peak table   
Select output items in the peak table.   
•  Peak time/Peak data 
•  Start time/End time 
•  Valley time/Valley data 
• Peak Area  
6) Include kinetics  
 
(d) Printer Font  
Click this button for changing the font to be used in the 
report.  
3.2  
						

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3.3  Defining Your Samples  
 
When “Use Sample Table” has been set for the Analysis Method, 
the samples must be defined before measurement.   
 
(1)  Open the monitor window and click the          button.   
 
(2)  When the Sample Table window in Fig. 3-8 appears, fill in 
the blank section.    For instance, if there are five samples, 
click the Insert button five times.   
 
 
 
Fig. 3-8    Sample Table Window 
 
 
(3)  Input the Sample, Comments and File Name for each 
sample.    The sample names input here appear on the 
monitor window also.     
Maximum number of characters to be input   
Sample : 20 characters  
Comments :  255 characters   
 
3.3  Defining Your Samples  
						

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(4)  When input is finished, check the entries and then click the 
OK button.   
If a sample table is not used, then click the          button.   
The window in Fig. 3-9 will appear.    Make entries for 
Sample and Comment.    For automatic saving, put a check 
mark in the “Auto file saving after measurement” box and 
specify a file name for saving.   
 
 
 
Fig. 3-9 
3.3  
						

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3.4    Conducting a Time Scan   
 
(1)  There are two methods of starting the scan.     
 
(a)  Select the Measure command from the 
Spectrophotometer menu.   
 
(b)  Click the     (measurement) button on the 
measurement toolbar.   
 
For halting the measurement midway, click the          button 
on the toolbar.   
 
(2)  Upon clicking the          button, the window shown in   
Fig. 3-10 appears when using a sample table.    Now click 
Yes.  
 
 
 
Fig. 3-10 
 
 
(3)  The dialog box as in Fig. 3-11 will open.    Set each sample 
and click OK.  
 
 
 
Fig. 3-11 
3.4    Conducting a Time Scan