Hitachi F 2500 Manual

							3 - 17 
(4)  When automatic saving is set, data will be saved after 
completion of measurement.    Upon opening its file, a data 
processing window will appear as in Fig. 3-12.     
Although a peak table can be displayed, simultaneous 
display of rate calculation (kinetics) result and peak table 
cannot be selected.    Fig. 3-12 shows a spectrum and the 
result of rate calculation.    This is selectable from the View 
menu.    Unless automatic saving is set, select the Save As 
command from the File menu and save data in file.   
 
 
 
 
Fig. 3-12    Data Processing Window (time scan) 
 
 
(a) Spectrum  
The spectrum of a measured sample is traced.   
 
(b)  Result of rate calculation   
The results of rate calculation (gradient, activity, R, R2) 
are displayed.    Start time and end time are settable by 
numeric input or clicking Set with the line cursor 
aligned.  After setting, click Update.  For rate 
calculation, refer to APPENDIX B.     
 
(c) Peak table  
Peak wavelength, valley wavelength, peak start/end 
wavelengths and peak area are displayed.   
(a) Spectrum 
(b) Result of rate 
calculation 3.4  
						

							3 - 18 
3.5  Data Printout  
 
Printout can be provided even when the function for automatic 
printout after measurement is not used.    The following two 
procedures are available.   
 
(1)  Select the Print command from the File menu.    Or click the   
        (print) button on the toolbar.    The field active on the 
data processing window (profiled in blue when clicked, field 
(a) in Fig. 3-12) will be printed.    The contents, that are 
displayed by selecting the Print Preview command from the 
File menu as shown in Fig. 3-13, will be printed.     
 
 
 
Fig. 3-13 
 
 
3.5  Data Printout  
						

							3 - 19 
(2)  Select the Report command in the Data menu.    Or click 
the    (report) button on the toolbar.  The Print Preview 
window will appear as shown in Fig. 3-14.    Then, click 
Print.    Select the items to be printed at the Report tab 
under Properties on the Edit menu.   
 
 
 
Fig. 3-14 
 
 
NOTE:  If characters overflow from the print frame in 
repeat measurement or in overlaying, then 
change the printing orientation from Portrait to 
Landscape.  
3.5  
						

							3 - 20 
3.6    Calculating the Phosphorescence Life   
 
This is the function for calculating phosphorescence life from the 
measurement result (phosphorescence attenuation curve).   
Replacing data value at T1 with I
0, I0/e time (T2) is found, and T1, 
T2 and τ (phosphorescence life) = T2 - T1 are displayed.   
Select the phosphorescence life command from the data (D) 
menu or click the          button on the Processing toolbar.     
A window as in Fig. 3-15 will appear.   
 
 
 
Fig. 3-15 
 
 
Move the line cursor to the start point (T1) of calculation by the 
mouse or arrow keys [←] and [→] on the keyboard.    Right-click 
the mouse at the start point.    Or input a numeral in the T1 field 
and click Calculate.    The result of calculation will be displayed.     
 
 
 
Fig. 3-16 
3.6    Calculating the Phosphorescence Life  
						

							3 - 21 
For printout of the calculation result, click Print.  For printout of 
the spectrum and calculation result, apply a check mark to “With 
spectrum record” and click Print.   
For scale expansion, specify the area to be enlarged with the 
mouse.  
Draw a rectangle with the mouse button held down as shown in 
Fig. 3-17.   
 
 
 
Fig. 3-17 
 
 
When releasing the mouse button, the specified area will be 
enlarged as shown in Fig. 3-18.   
 
 
 
Fig. 3-18 
 
 
Double-clicking on the spectrum selects auto scale.   
 
3.6  
						

							4 - 1 
4.  PHOTOMETRY 
 
 
4.1  Flow of Operation 
 
This section explains the method of determining sample 
concentration with a calibration curve prepared.    Following is 
the operational flow. 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Fig. 4-1    Operational Flow (photometry) 
 
 
 
End 
Start 
Method setting 
Click  button.  
 Photometric method
 Measuring 
wavelength 
 Standard table 
 
Sample name/comment input
Click  button.  
Measurement 
Measurement of standard 
and sample 
Print  (See page 4-2.) 
(See page 4-18.) 
 
(See page 4-19.)   
 Click 
 (measurement) 
button.  
Click 
 button.  
(See page 4-24.)     
 
 
4.1  
						

							4 - 2 
4.2    Creating an Analysis Method   
 
Set the analytical conditions (method) prior to measurement.   
From the Edit menu, select the Method command or click the 
button.    The dialog box shown in Fig. 4-2 will appear.   
 
 
 
Fig. 4-2  Analysis Method 
 
 
The following tabs are provided on the Analysis Method window 
for the photometry mode.    These tabs are explained on the 
subsequent pages.   
• General 
• Quantitation 
• Instrument 
• Standards 
• Monitor 
• Report  
 
4.2    Creating an Analysis Method  
						

							4 - 3 
(1) General  
 
The parameters shown in Fig. 4-3 are provided under the 
General tab.   
 
 
 
Fig. 4-3  General Tab 
 
 
(a) Measurement (mode)  
Select a measurement mode from among the four 
listed below.   
• Wavelength scan  
• Time scan  
• Photometry  
•  3-D Scan (displayed when the ‘3-D Scan program’ 
(option) is installed in combination with Model   
F-2500)  
Here, select “Photometry.”    For Wavelength scan, 
Time scan and 3-D Scan, refer to Sections 2, 3 and 5.   
 
(b) Operator  
Enter an operator name.     
 
(c) Instrument  
The model of the connected instrument is indicated.   
 
(d)  Use sample table (Set measurement sample) 
Apply a check mark here when a sample table is used.     
 
4.2  
						

							4 - 4 
(e) Sampling  
• Standard  
When sample is exchanged manually.   
 
(f) Comments  
Enter a description or notes on measuring conditions.   
 
(g) Load  
Click this button to read out saved analytical conditions.   
The file opening window appears upon clicking, so 
select an analytical conditions file.   
 
(h) Save  
By clicking this button after setting the parameters for 
all six tabs, the analytical conditions are saved.   
Clicking this button displays the “Save As” window in 
creation of a new analytical conditions file.    So input a 
file name.   
 
(i) Save As  
Click this button for renaming the set conditions for 
saving.   
 
(2) Quantitation  
 
Upon selecting the Quantitation tab, the window shown in 
Fig. 4-4 appears.   
 
 
 
Fig. 4-4  Quantitation Tab 
 
4.2    Creating an Analysis Method  
						

							4 - 5 
(a) Quantitation type  
Select the method of creating a calibration curve.   
Wavelength, Peak area, Peak height or Derivative 
method is selectable.   
Spectra measured by the Peak area, Peak height and 
Derivative method can be checked.   
 
1) Wavelength  
Upon selecting “None” for calibration curve type in 
this quantitation method, up to 6 wavelengths can 
be set.    When any other than “None” is selected 
for quantitation type, up to 3 wavelengths can be 
set.   
(i)  One wavelength calculation   
This is the most generally used method.     
As shown in Fig. 4-5 (a), one wavelength is 
specified, and the concentration is 
determined from the data I
1 at that time.   
(ii)  Two wavelength calculation   
As shown in Fig. 4-5 (b), assuming I
1 and I2 
are the absorbance values at two different 
wavelengths, concentration is calculated 
through determination of I by the equation 
below.  
I = I
2 - I1  
(iii)  Three wavelength calculation   
As shown in Fig. 4-5 (c), assuming    I
1, I2 and 
I
3 are the data values at three different 
wavelengths, concentration is calculated 
through determination of I by the equation 
below.    Photometry is made in the order of 1, 
2, 3. 
 
I = I
2 -   
 
But the following relation must hold:   
1 < 2 < 3 or 1 > 2 > 3   1 - 3  1 - 2 × I
3 + 2 - 3 × I1 
4.2