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Hitachi F 2500 Manual

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    							6 - 15 
    (2)  Open a file to be overlaid to display its data.   
     
     
     
    Fig. 6-15 
     
     
    Drag the blue profile of the displayed spectrum so that the 
    mouse pointer comes onto the target spectrum.    When the 
    mouse pointer changes as shown in Fig. 6-16, drop the 
    blue profile.   
     
     
     
    Fig. 6-16 
     
     
    (3)  Open a file as in (2) above.    Click the spectrum to be 
    overlaid and press Ctrl+C on the keyboard or select the 
    Copy (c) command from the Edit (E) menu.    Then click the 
    target spectrum and paste the first spectrum on it by Ctrl+V.  
    The spectra are now overlaid.   
     
    6.10  
    						
    							6 - 16 
    (a) Deletion from Overlaid Display  
    Select the Overlay command from the Processing 
    menu.    In case you want to delete the spectrum 
    having sample name “Filter 02,” select the sample 
    name and click Delete.   
     
     
     
    Fig. 6-17 
     
     
    (b) Display of Selected Spectrum Alone from Overlaid 
    Spectra  
    Click     (Display spectrum) on the Spectrum toolbar.  
     
     
     
    Fig. 6-18 
     
     
    Click the spectrum not to be displayed.   
    Click OK.    In this case, spectra Nos. 1 and 2 will be 
    displayed.    Printout of this data covers not the spectra, 
    but the sample names, analytical conditions, etc.   
    After file saving in the current state, 4 spectra will be 
    displayed upon loading the file again.   
    To be displayed   
    Not to be displayed   
     
    6.10    Overlaid Display of Spectra  
    						
    							6 - 17 
    6.11    Arithmetic Operation between Spectra   
     
    Shown here is an example of obtaining the difference between 
    two overlaid spectra.     
    Display the spectrum for arithmetic operation.    Now, click the 
    Arithmetic (A) command on the Data (D) menu or click the 
    (spectral calculation) on the Processing toolbar.    A window as in 
    Fig. 6-19 will open.     
     
     
     
     
     
    Fig. 6-19 
     
     
    (1)  The sample name of the file open in the window (file name 
    in parentheses) is indicated.    Select the file for arithmetic 
    operation.   
     
    (2)  The spectrum selected in (1) is displayed here.   
     
    (3)  A factor for the spectrum selected in (1) is settable.     
    For multiplying the spectrum by a factor, click Calculate 
    here.  
     
    (4)  Apply a check mark here for arithmetic operation between 
    two spectra.   
    As a result, the window as in Fig. 6-20 appears.   
    (2) Spectrum 
    (1) Sample  name (3) Factor  input (4) Spectral  calculation 
    ON/OFF  
    6.11  
    						
    							6 - 18 
     
     
     
     
    Fig. 6-20 
     
     
    (5)  Another sample name (file name in parentheses) is 
    indicated.    Select the file for arithmetic operation.     
     
    (6)  The spectrum selected in (5) is displayed.   
     
    (7)  Set the factor for the spectrum selected in (5).   
     
    (8)  Select a mathematical operator for the two spectra.     
    +:  Addition, -:  Subtraction, *:  Multiplication, /:  Division  
     
    After setting, click Calculate.    The result of arithmetic operation 
    is displayed as shown in Fig. 6-21.     
     
    (6) Spectrum 
    (5) Sample  name (7) Factor  input  (8) Mathematical  operator  6.11    Arithmetic Operation between Spectra  
    						
    							6 - 19 
     
     
     
     
    Fig. 6-21    After Arithmetic Operation 
     
     
    Click Save As, and the result of calculation can be saved in file.   
    Result of arithmetic operation 6.11  
    						
    							6 - 20 
    6.12  Area Calculation  
     
    An area value is obtainable by integration in the specified range 
    of the presently displayed spectrum.     
    Select the Integration (I) command from the Data (D) menu or 
    click the          (area calculation) button on the Processing toolbar.   
    The window as in Fig. 6-22 will appear.     
     
     
     
    Fig. 6-22 
     
     
    Click Start and input the range for area calculation.    Or move 
    and right-click the cursor, and a value will be entered.    In the 
    same way, enter the end wavelength.    After entry, click 
    Calculate.    The result will be displayed.   
    For printout, click Print.    In order to print a spectrum and the 
    result of calculation, apply a check mark to “With spectrum 
    record” and click Print.   
    When clicking the OK button after calculation, its result will be 
    saved.    Operation will be reset by closing the data processing 
    window or clicking the          button on the Processing toolbar.     
    Clicking Cancel closes the window.   
     
    6.12  Area Calculation  
    						
    							6 - 21 
    For scale expansion, specify the area to be expanded with the 
    mouse.    Draw a rectangle with the mouse button held down.     
     
     
     
    Fig. 6-23 
     
     
    Then, release the button, and the specified area will be 
    expanded as shown in Fig. 6-24.     
     
     
     
    Fig. 6-24 
     
     
    Double clicking the right mouse button returns the scale to the 
    original one.   
    6.12  
    						
    							6 - 22 
    The principle of calculation is shown below.   
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
    Fig.6-25 
     
     
    The elements (D
    1 to Dn) of a spectrum are subjected to the 
    following calculation for obtaining the area value.     
     
    S3 =  {D
    2 + D3 + … Dn-2 + Dn-1 + D1 + Dn}/2∗∆λ 
    S2 =  (D
    1 + Dn) ∗ {∆λ∗ (n-1)} 
    (D
    1 + Dn) ∗ (Section start wavelength (WL1)) –   
    (Section end wavelength (WL
    2)) 
    S1 = S3 – S2 
    D
    1 :  Spectral data at section start wavelength   
    D
    n :  Spectral data at section end wavelength   
    ∆λ :  Sampling interval   
     
    In the time scan mode, change as follows.   
    Start wavelength (WL
    1) → end time (T2)  
    End wavelength (WL
    2) → start time (T1)  
    6.12  Area Calculation 
     
     
    Section start 
    wavelength   Section end 
    wavelength  
     Specified range   
    Dn D
    n - 1
    D2 
    D
    1 
    ∆
    2 
    WL
    1
    S2 S
    1 
    WL
    2WL1
    Sb  S
    a 
    WL
    2  S
    c 
    S
    d  
    						
    							6 - 23 
    6.13    Transfer of Data to Microsoft(R) Excel   
     
    Measured data can be transferred to a worksheet of Microsoft(R) 
    Excel.   
    Procedure is given below.   
     
    (1)  Click the          (properties) button and select the Report tab.   
    A window as in Fig. 6-26 will open.    In Print Method, select 
    “Use Microsoft(R) Excel.”    Also select the data to be 
    transferred (peak table and data list in case of wavelength 
    scan).  Then, click OK.  
     
     
     
    Fig. 6-26 
     
     
    (2)  Click the          (Report) button and the Microsoft(R) Excel 
    will automatically start to read the data.    The subsequent 
    procedure concerns the Microsoft(R) Excel operation.   
     
     
     
    Fig. 6-27 
    6.13  
    						
    							6 - 24 
    (3)  Data can be saved with extension code xls of Microsoft® 
    Excel. 
    Select File - Save Copy As and enter a file name.     
     
     
     
    Fig. 6-28 
     
     
    (4)  Upon closing the Microsoft® Excel, the window in Fig. 6-29 
    appears.    Now close this window.    The original data 
    processing window will appear again.   
     
     
     
    Fig. 6-29 
    6.13    Transfer of Data to Microsoft(R) Excel  
    						
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