Hitachi F 2500 Manual

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(3) General  
 
The parameters shown in Fig. 5-3 are provided under the 
General tab.   
 
 
 
Fig. 5-3  General Tab 
 
 
(a) Measurement (mode)  
Select a measurement mode from among the four 
listed below.   
 Wavelength scan  
 Time scan  
 Photometry  
 3-D Scan  
Here, select “3-D Scan.”    For Wavelength scan, time 
scan and Photometry, refer to Sections 2, 3 and 4.   
 
(b) Operator  
Enter an operator name.     
 
(c) Instrument  
The model of the connected instrument is indicated.   
 
(d) Sampling 
 Standard  
When sample is exchanged manually.   
 
(e) Comments  
Enter a description or notes on measuring conditions.   
 
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(f) Load  
Click this button to read out saved analytical conditions.   
The file opening window appears upon clicking, so 
select an analytical conditions file.   
 
(g) Save  
By clicking this button after setting the parameters for 
all five tabs, the analytical conditions are saved.   
Clicking this button overwrites parameters on the 
existing ones.   
 
(h) Save As  
Click this button for renaming the set conditions for 
saving.    The “Save As” window will appear, so enter a 
file name.   
 
(4) Instrument  
 
This window appears when 3-D Scan is selected for the 
Measurement under the General tab of the Analysis 
Method window.    When you click the Instrument tab, the 
window in Fig. 5-4 appears.   
 
 
 
Fig. 5-4  Instrument Tab 
5.2    Creating an Analysis Method  
						

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(a) Data mode  
Select either of the following data modes.   
The Phosphorescence mode is for acquiring only 
phosphorescence after extinction of excitation beam 
while turning a chopper on the excitation side.     
 Fluorescence  
  Phosphorescence (with Model F-4500)   
 
(b)  EX Start WL   
This is the start wavelength for wavelength scan on the 
excitation side.    Input the shorter wavelength side of 
measuring wavelength range.   
 
Model Input Range  
F-4500  0, 200 to 850 nm 
F-2500  0, 200 to 750 nm 
 
 
(c)  EX End WL   
This is the end wavelength for wavelength scan on the 
excitation side.    Input the longer wavelength side of 
measuring wavelength range.   
 
Model Input Range  
F-4500  0, 200 to 900 nm 
F-2500  0, 200 to 800 nm 
 
 
(d)  EX Sampling interval   
In the 3-dimensional measurement mode, emission 
spectrum measurement is repeated while shifting the 
excitation wavelength.    Therefore, a shorter sampling 
interval on the excitation side will result in a longer 
measurement time.   
Input range:    1 to 50 nm   
 
(e)  EM Start WL   
This is the start wavelength for wavelength scan on the 
emission side.    Input the shorter wavelength side of 
measuring wavelength range.     
 
Model Input Range  
F-4500  0, 200 to 850 nm 
F-2500  0, 200 to 750 nm 
 
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(f)  EM End WL   
This is the end wavelength for wavelength scan on the 
emission side.    Input the longer wavelength side of 
measuring wavelength range.     
 
Model Input Range  
F-4500  0, 200 to 900 nm 
F-2500  0, 200 to 800 nm  
 
 
(g)  EM Sampling interval   
In the 3-D Scan mode, emission spectrum 
measurement is repeated while shifting the excitation 
wavelength.    Therefore, a shorter sampling interval on 
the emission side will result in a longer measurement 
time.  
Input range:    1 to 10 nm   
 
(h) Scan speed  
Set a scan speed.  (Unit:  nm/min)  
 
F-4500 F-2500 
30000 3000 
12000 1500 
2400 300 
1200 60 
300 15 
60  
15  
 
 
(i) EX Slit  
Select a slit width for the excitation side.    Unit:    nm   
 
F-4500 F-2500 
1.0 2.5 
2.5 5.0 
5.0 10.0 
10.0 20.0 
20.0  
 
 
5.2    Creating an Analysis Method  
						

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(j) EM Slit  
Select a slit width for the emission side.    Unit:    nm   
 
F-4500 F-2500 
1.0 2.5 
2.5 5.0 
5.0 10.0 
10.0 20.0 
20.0  
 
 
(k) PMT Voltage  
A function for controlling the voltage of the 
photomultiplier detector (photomultiplier tube).     
Select one of the following values.    Changing from 
400 V to 700 V increases the data value about two 
digits.   
  950 V (only with F-4500)   
 700 V  
 400 V 
 
(l) Response  
Select a response time from the following.   
 
F-4500 F-2500 
0.04 0.04 
0.01 0.08 
0.05 0.4 
0.1 2 
0.5 8 
2.0 Auto 
8.0  
Auto  
 
 
By selecting Auto, a response time is set automatically 
according to scan speed.    Select Auto usually.   
 
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(m) Corrected spectra  
A function for determining the spectrum inherent to a 
sample by correcting the photometer wavelength 
characteristic using the saved instrument parameters, 
following measurement with the instrument parameters 
for photometer control.    If there are no instrument 
parameters for the wavelength range to be measured, 
only the wavelength range that allows correction is 
scanned.    And if there is no wavelength area 
measurable, an error will occur.     
For using this function, a Spectra Correction Parts Set 
(P/N 251-0126) is required.    The Instrument 
Response command is provided in the Utility menu for 
operating this function.    When this setting is at ON, 
“Corrected spectra” appears on the monitor window.   
(See Fig. 1-26 in 1.6.)   
 
(n)  Shutter control (only with F-4500)   
The shutter can be automatically closed in other than 
measurement for suppressing sample deterioration 
due to the energy of excitation beam and opened when 
measurement starts.    When you put a check mark at 
the head, the shutter will close and open at start of 
measurement.    The shutter will close again when 
measuring wavelength begins returning to the start 
wavelength after measurement in the wavelength scan 
mode.   
 
5.2    Creating an Analysis Method  
						

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(5) Monitor  
 
When selecting the Monitor tab, the window in Fig. 5-5 
appears.   
 
 
 
Fig. 5-5  Monitor Tab 
 
Assign the following parameters.   
 
(a)  Y-Axis Max (maximum)   
Input a maximum value for Y-axis on the monitor 
window.  
 
(b)  Y-Axis Min (minimum)   
Input a minimum value for Y-axis on the monitor 
window.  
 
(c) Contour interval  
Function for setting intervals of drawing contour lines.   
Input range:    0.01 to 1000   
 
(d)  Open data processing window after data acquisition   
Select whether or not to conduct data processing after 
sample measurement.    When selected (check mark is 
applied), an icon is displayed for the data processing 
window at the end of measurement.    And by opening 
this icon, data processing such as peak detection is 
available.  
 
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NOTE:  When the remaining memory capacity of PC 
in operation is very small (system resource is 
less than 20%), the data processing window 
will not open even if this item is turned ON.   
Either close the presently opened data 
processing window or restart the FL 
Solutions program.   
 
(e)  Print report after data acquisition   
Select whether or not to print a report after sample 
measurement.    When selected (check mark is 
applied), printing will be made automatically after 
measurement.    The items set under the Report tab 
will be printed.    When this item is not selected, data 
processing can be made by reading out a file via File-
Open.  
 
(6) Processing  
 
On selection of the Processing tab, the window in Fig. 5-6 
will be displayed.   
 
 
 
Fig. 5-6  Processing Tab 
 
 
(a) Peak Finding  
Select a peak detection method indicated in the data 
processing window.   
 
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(b) Threshold  
Set a detection limit for the photometric value axis of 
peak and valley.    Peaks or valleys below the setting 
will not be detected.    Input range is 0.001 to 1000.     
 
(c) Sensitivity  
Select the number of data points in the horizontal axis 
direction (abscissa).    Select a sensitivity of “1” for 
detecting sharp peaks.    Or select “8” for detecting 
broad peaks.   
 1  
 2 
 4 
 8 
 
(7) Report  
 
Select the Report tab, and the window in Fig. 5-7 will 
appear.  
 
 
 
Fig. 5-7  Report Tab 
 
 
(8) Output 
 
(a) Print Report  
A report will be printed.    For printing method, refer to 
5.7.  
 
(b)  Use Microsoft® Excel   
Data will be transferred to Microsoft® Excel.     
For transfer method, refer to 6.13.   
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(c)  Use print generator sheet   
Select this method for using the report generator 
(option).    For usage, refer to the instruction manual of 
the report generator.   
 
(9) Orientation  
Selectable when Report is chosen for Print Method.   
Select a printing orientation.    Either Portrait or Landscape 
orientation is selectable.    When the Landscape orientation 
is selected, date and method can be printed.   
 
(a)  Printable (transferable) items   
1) Include date  
2) Include instrument 
3) Include graph  
 Contour  
 Bird’s-eye view  
  Contour and bird’s-eye view 
  Contour and Spectrum   
4)  Include peak table   
 
(b) Printer Font  
Click this button for changing the font to be used in the 
report.  
5.2    Creating an Analysis Method